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U.S. Department of Transportation U.S. Department of Transportation Icon United States Department of Transportation United States Department of Transportation

Drug and Alcohol Testing Brochure for Drivers

The U.S. Department of Transportation (USDOT) drug and alcohol testing regulations are contained in 49 CFR part 40; the drug and alcohol testing regulations for entities regulated by the Federal Motor Carrier Safety Administration (FMCSA) are contained in 49 CFR part 382.

This brochure summarizes the regulations as they apply to commercial driver’s license (CDL) drivers, and is intended to better inform drivers about their rights and obligations as participants in a USDOT drug and alcohol testing program.

This brochure was updated in June 2024 to incorporate regulations regarding the Drug and Alcohol Clearinghouse and the potential loss of commercial driving privileges if a CDL driver has a drug and alcohol program violation entered in their Clearinghouse record.

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Last updated: Tuesday, July 16, 2024