Substance Abuse Professionals

Substance Abuse Professionals (SAPs) are licensed or certified professionals that have knowledge not only of the diagnosis and treatment of alcohol and controlled substances related disorders, but also of DOT drug and alcohol testing and return-to-duty processes as required by §40.281. A DOT-...

Drug Testing Laboratories

Drug testing laboratories must be certified by the Department of Health and Human Services. These laboratories receive, analyze and report laboratory confirmed results only to MROs. Regulations concerning drug testing laboratories are found in 49 CFR Part 40 Subpart F, Subpart Q and Subpart R...

Medical Review Officer

Medical Review Officers (MRO) are licensed physicians who have been trained and certified to review the lab results and to validate whether a test is positive. MROs receive laboratory confirmed urine drug test results; determine whether there is a legitimate medical explanation for a laboratory...

Urine Collectors

Urine collectors collect specimens using Part 40 procedures, ship the specimens to Department of Health and Human Services (HHS) certified laboratories for analysis, and distribute copies of the Federal Drug Testing Custody and Control Form (CCF) to thelaboratory, medical review officer,...

Best Practices for DOT Random Drug and Alcohol Testing

The purpose of this publication is to help DOT covered employers and service agents in implementing and evaluating their own random tes ting programs. While DOT regulations serve as a mandatory minimum and do not prevent additional practices that serve the effectiveness of a testing program, don...

Drug and Alcohol Testing Brochure for Drivers

The U.S. Department of Transportation (U.S. DOT) drug and alcohol testing regulations are contained in 49 CFR part 40; the drug and alcohol testing regulations for entities regulated by the Federal Motor Carrier Safety Administration (FMCSA) are contained in 49 CFR part 382. This brochure...

Drug and Alcohol Testing MIS Data Collection Form Instructions

Drug and Alcohol Testing MIS Data Collection Form

What are Service Agents?

A service agent is any person or entity, other than an employee of the motor carrier, used to help implement the DOT/FMCSA drug and alcohol testing regulations. These might include a urine collector, a breath alcohol technician, a screening test technician, a laboratory, a medical review officer...

Employers Resources and Downloads

Employers seeking additional information on DOT drug and alcohol testing rules are encouraged to use the resources listed on this page.